Department of Biochemistry

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Browsing Department of Biochemistry by Author "Didas, Mushabe"

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    Comparative Assessment of Red Blood Cell Morphology in Anaemic Children
    (International Journal of Sciences, 2020) Samuel, Mwesige; Clement, Munguiko; Didas, Mushabe
    Abstract Anaemia (haemoglobin level < 11 g/dl) is a health burden among preschool children and women of child bearing age and affects over 27% of the World population. Anaemia results from reduction in the functional haemoglobin or red blood cell numbers or mass leading to decreased oxygen carrying capacity characterized by clinical features such as; skin pallor, fatigue, shortness of breath, congestive heart failure, jaundice and tachycardia. Evaluating and interpreting red blood cell morphology provides key information in the differential diagnosis of Anaemia. However, the current standard Peripheral thin blood method of assessing red blood cell morphology is highly technical and time consuming. There was need to carry out assessment into the alternative Automated Complete Blood Count method to aid in the selection of the reliable assay. The objective of the study was to compare between Peripheral blood thin film and Automated Complete Blood Count morphologically classified Anaemia in children. The study was cross-sectional and employed simple random sampling technique. Blood samples were obtained from the participants, assessed for red blood cell morphology by Automated Complete Blood Count and Peripheral thin blood film. Data was analyzed using SPSS and a paired t-test used to test for the statistical significance. Results show no significant difference in the scores for Peripheral thin blood film (M=25.5, SD=11.82) and Automated Complete Blood Count (M=25.5, SD=12.66) t (3) =0.00, p =1.000. Automated Complete Blood Count is a method of choice in assessing red blood cell morphology and evaluating Anaemia. The study recommends assessment into various Automated Complete Blood Count models available in the market to aid in the selection of most reliable one.
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    The Silent Anaemia Epidemic in Children
    (International Journal of Innovative Science and Research Technology, 2020-05) Samuel, Mwesige; Annete, Nankwanga; Didas, Mushabe
    Abstract:- Anaemia (haemoglobin level < 11 g/dl) is a silent epidemic that affects about 293.1 million children aged <5 years worldwide with 28.5% of those located in sub-Sahara Africa. Child hood Anaemia is associated with poor cognition, reduced growth, problems with immune function and ultimately decreased survival. Uganda’s childhood Anaemia is insidious, multifactorial and undefined. The objectives of the study were to determine the prevalence, severity and leading cause of Anaemia amongst children ≤ 12 years attending Virika hospital. The cross-sectional study employed both qualitative and semi-quantitative methods. Participants were sampled by convenience and simple random techniques. Parents were given short interviews to obtain participants demographic and necessary information. Blood, stool and anthropometric measurements were obtained from the participants and tested for complete blood count, malaria, HIV, sickle cell disease, helminthes and malnutrition respectively. Data was analyzed using Excel computer program. Results show that Prevalence of Anaemia in children is high, presenting mostly in moderate form. Malnutrition plays a major role in the development of Anaemia. Nutritional support and awareness are key aspects in prevention of Anaemia development and progression. Other control interventions may include provision of improved diagnostic tests, optimizing compliance, appropriating referral practices, implementation of standard treatment guidelines and research.